Spe Cartridge
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 Varian Chem elut SPE cartridge column C18 Extube 1 GRM  US $57.00
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 Varian Chem elut SPE cartridge column C18 Extube 1 ml US $45.00
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 Varian Bond Elut SPE cartridge column CN E 100 mg 1 ml US $14.00
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How do I work out the orignial concentration?
I pipetted 5ml of sample and added 2mls of 0.1M phosphate buffer (pH6.0). I then eluted 3mls of the sample into a prepared SPE cartridge.
From my chromatographic results obtained from the acid/neutral fraction analyzed I need to calculate the original concentration of Hydrocortison, Corticoserone and 1-hydroxyprogesterone in the sample.
To do this, you need to calculate the aliquoting factor or the dilution factor. Then apply this to the value you obtained for each analyte. So, 5 ml of original sample diluted to 7 mls represents a 1.4 fold dilution or a 40% dilution. At this point, I'm not sure what you then did. You state that you eluted 3 mls into a cartridge, but eluted from what, a column? If so, how much was applied to the column? Whatever you did, just work backwards and keep track of the dilutions, etc.
LC-SPE-MS/NMR cartridge handling robot
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