Fluorescence Microscope
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![]() Nikon DM455 Microscope Fluorescence Filter Block BV US $49.99
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Image looks like a neative stain in this microscope?
Image looks a negative stain in this microscope:
fluorescence microscope
phase-contrast microscope
darkfield microscope
DIC microscope
none of these
phase-contrast microscope
High content live cell imaging for the discovery of new antimalarial marine natural products (biomedcentral)
Background: The human malaria parasite remains a burden in developing nations.
It is responsible for up to one million deaths a year, a number that could
rise due to increasing multi-drug resistance to all antimalarial drugs
currently available. Therefore, there is an urgent need for the discovery of
new drug therapies. Recently, our laboratory developed a simple one-step
fluorescence-based live cell-imaging assay to integrate the complex biology of
the human malaria parasite into drug discovery. Here we used our newly
developed live cell-imaging platform to discover novel marine natural products
and their cellular phenotypic effects against the most lethal malaria
parasite, Plasmodium falciparum. Methods: A high content live cell imaging
platform was used to screen marine extracts effects on malaria. Parasites were
grown in vitro in the presence of extracts, stained with RNA sensitive dye,
and imaged at timed intervals with the BD Pathway HT automated confocal
microscope. Results: Image analysis validated our new methodology at a larger
scale level and revealed potential antimalarial activity of selected extracts
with a minimal cytotoxic effect on host red blood cells. To further validate
our assay, we investigated parasite's phenotypes when incubated with the
purified bioactive natural product bromophycolide A. ...
Intro to Fluorescence Microscopy
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